Results/Conclusions Limited germination was observed in all plots, with <20% of any species of either seed mix exhibiting germination. In a complementary greenhouse study, seeds of each species from the high diversity seed mix (34 total species) were seeded into freshly collected field soil from each OWB eradication treatment (single, multiple, and combined treatments of glyphosate and burning) and percent germination was assessed 1,2,3,4, and 7 weeks following seeding. Soil from OWB monocultures sites significantly reduced germination of all native species tested. Soil collected from OWB eradication sites (receiving glyphosate and burning) were generally characterized by increased germination of native seeds, as compared to OWB monoculture sites. However, amending the field soils with native prairie soil improved germination of all treatment soils, with a 10-fold increase in germination observed for many of the native species. This study indicates that successful remediation of OWB monocultures to native plant communities may be improved with amendments of native prairie soils, thereby restoring soil biotic communities and function.