Results/Conclusions All removal methods significantly decreased star thistle biomass. Local eradication (no recurring individuals, no star thistle germination from seedbank) was achieved in some patches of every removal treatment, but not in all patches of any treatment. Star thistle removal significantly decreased downstream colonization of new star thistle patches. Native and perennial species increased in biomass when star thistle declined, but non-native annuals also increased. The most cost-effective method was mowing the star thistle prior to seed set. This study provides support for using mechanical control of star thistle in areas where herbicide use is undesirable.