Results/Conclusions Our results indicate that there were no differences in the density or family richness of macroinvertebrates, but family diversity was higher in non stocked streams. Results of ordination analyses suggest that macroinvertebrate assemblages are affected by location in the landscape (pairs are similar) and the presence of stocked fish. Fish biomass was marginally higher in non stocked streams, and we identified differences in size distributions of some species. Against a background of considerable variation among streams, our study measured differences in macroinvertebrate and fish assemblages that appear to be related to trout stocking. This and other studies support the need for further research related to impacts of widespread stocking practices.