Print PageTsingtau Lily (Lilium tsingtauense Gilg = Lilium miquelianum Makino) is narrowly distributed species limited to certain areas of China and Korea. Genetic diversity and population structure of 12 populations of Tsingtau Lily (Lilium tsingtauense Gilg; Liliaceae) in China and Korea were investigated using Inter-Simple Sequence Repeat (ISSR) molecular markers and morphological characters.
Results/Conclusions
Analysis based on 65 ISSR marker-fragments generated by 6 primers from 648 individuals showed that there exists a high level of genetic variability in L. tsingtauense. The percentage of polymorphic loci, effective number of alleles, average expected heterozygosities and Shannon’s information index were 72.31%, 1.437, 0.231 and 0.369, respectively. Genetic differentiation among populations (GST=0.144) demonstrated considerable genetic variation within populations (85.6%) rather than among populations (14.4%). Gene flow (Nm=1.487) was considerable at species level. Genetic diversity in Korea was much higher than that in China. Genetic distance and geographic distance correlated significantly. Cluster analysis showed that groupings of the accessions according to ISSR data correlated well with their geographic region and the populations in China differentiated from their Korean counterparts. Population CL7 appeared micro-scaled spatial structured by autocorrelation coefficients and revealed the case of random spatial distribution over a distance of 400cm within the confidence interval of 95%. The morphological characters showed high correlation with ISSR parameters. No altitudinal differences were found with morphological characters or ISSR parameters. There was a low genetic differentiation among different age classes of L. tsingtauence in China (GST=0.013) and a high gene flow among different age classes (Nm=18.344).
