OOS 34 - Microbial Ecology Using Metagenomics

Thursday, August 11, 2011: 8:00 AM-11:30 AM
17A, Austin Convention Center
Organizer: Emma L. Aronson
Co-organizer: Naupaka Zimmerman
Moderator: Aurora MacRae-Crerar
Metagenomics, broadly speaking, is the study of pooled genetic material taken directly from environmental samples. This allows for the simultaneous characterization of a community of organisms present in a sample and can provide inference as to their metabolic capabilities as well. The current range of methods used for metagenomic analysis includes: clone library creation; DNA or RNA microarrays, such as the Phylochip or Geochip, which include probes for most previously known genetic information; and whole environmental sequencing of either entire genomes or certain subsets, such as functional genes or conserved 16S regions through high-throughput techniques such as 454 pyrosequencing. This session aims to bring together researchers using the methods described above to study the microbiomes (including one or all of bacteria, archaea and fungi) of all types of systems, and to provide a synthesis of how ecologists are using these tools to increase their understanding of the structure and functioning of ecosystems. Confirmed speakers will present research on techniques including GeoChip, PhyloChip, barcoded pyrosequencing, clone library construction and shotgun sequencing to study systems ranging from the human microbiome to hot springs and the soil under our feet. The scale of observation in these systems varies between thousands of kilometers in an open landscape to millimeters in the human mouth. Using these innovative technologies, the research presented in this session furthers our understanding about the ecological interactions between organisms, between organisms and their environment, and about the intersection of biodiversity and ecosystem function on multiple scales and in vastly different ecosystems.
8:00 AM
Linking the microbial community structures and functions of hot spring phototrophic bacterial mats with comparative metagenomics
Christian G. Klatt, Montana State University; Mary N. Parenteau, NASA Ames Research Center; Sarah M. Boomer, Western Oregon University; Zackary Jay, Montana State University; Scott R. Miller, University of Montana; Jason M. Wood, Montana State University; Donald A. Bryant, The Pennsylvania State University; William P. Inskeep, Montana State University; David M. Ward, Montana State University
8:20 AM
Metagenomics of dormancy and implications for the maintenance of microbial diversity
Jay T. Lennon, Indiana University; Stuart E. Jones, University of Notre Dame
9:00 AM
Cross-site comparison of three terrestrial-subsurface bacterial assemblages using PhyloChip and GeoChip microarrays
Rebecca A. Daly, University of California, Berkeley; Damon C. Bradbury, University of California, Berkeley; Hsiao Chien Lim, Lawrence Berkeley National Laboratory; Ping Zhang, Institute for Environmental Genomics; Catherine A. Osborne, University of California, Berkeley; Jiamin Wan, Lawrence Berkeley National Laboratory; Tetsu K. Tokunaga, Lawrence Berkeley National Laboratory; Zhili He, University of Oklahoma; Jizhong Zhou, University of Oklahoma; Eoin L. Brodie, University of California, Berkeley; Mary K. Firestone, University of California, Berkeley
9:20 AM
Investigation of the microbial community and methane cycle of a pine forest soil using Phylochips and qPCR
Emma L. Aronson, University of Pennsylvania; Eric A. Dubinsky, Lawrence Berkeley National Laboratory; Gary L. Andersen, Lawrence Berkeley National Laboratory; Brent Helliker, University of Pennsylvania
9:40 AM
9:50 AM
Fungals in the Jungles: Endophytic fungal diversity at the landscape scale
Naupaka Zimmerman, University of Arizona; Peter M. Vitousek, Stanford University
10:30 AM
How will expanding taxonomic and functional molecular characterization of complex soil microbial assemblages advance our understanding of microbial ecology?
Mary K. Firestone, University of California, Berkeley; Damon C. Bradbury, University of California, Berkeley; Rebecca Daly, University of California, Berkeley; Kristen DeAngelis, University of Massachusetts, Amherst; Sarah A. Placella, University of California
10:50 AM
Response of nutrient cycle functional nutrient genes to prolonged no-till practices and crop rotations in  a farming system experiment in North Carolina
Sanghoon Kang, University of Houston, Clear Lake; Bo Liu, North Carolina State University; Frank Louws, North Carolina State University; Jizhong Zhou, University of Oklahoma
11:10 AM
Global biodiversity and biogeography of marine bacteria
Joshua Ladau, Gladstone Institutes, University of California San Francisco; Thomas J. Sharpton, Gladstone Institutes; Guillaume Jospin, UC Davis Genome Center; Steven W. Kembel, Université du Québec à Montréal; James O'Dwyer, Santa Fe Institute; Alex Koeppel, University of Virginia; Jessica L. Green, University of Oregon; Katherine S. Pollard, Gladstone Institutes, University of California San Francisco
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