OPS 5-15
Identifying cryptic lineages within an endemic frog genus (Indirana) in the Western Ghats

Wednesday, August 12, 2015
Exhibit Hall, Baltimore Convention Center
Vijay Ramesh, Department of Ecology, Evolution & Environmental Biology, Columbia University, New York, NY
Vijayakumar SP, Centre for Ecological Sciences, Indian Institute of Science
Kartik Shanker, Centre for Ecological Sciences, Indian Institute of Science
Trisha Gopalakrishna, Nicholas School of the Environment, Duke University, Durham, NC
Background/Question/Methods

A large number of species in the tropics are awaiting discovery, many due to their crypticity ie. lack of morphological difference. We tested for crypticity in a frog genus, Indirana, endemic to the Western Ghats of peninsular India. We updated a published phylogeny using increased taxon and spatial sampling, and by examining the relationship between genetic and morphological divergence among close relatives. Initially, we delimited potential lineages using a 16S rRNA haplotype tree and then applied, multiple criteria including haplotype clusters in a multi-gene tree, genetic distances and geographical range separation. We then examined pairs of potential lineages with differing levels of genetic divergence in multivariate morphological space.

Results/Conclusions

Our results suggest that there are 19 – 21 potential lineages in Indirana, which is an increase of 70 – 90 % from earlier estimates. Several lineages within Indirana were cryptic and could not be distinguished on the basis of morphology, and the potential lineages identified await formal description as species.